JCS -- Champion et al. 117 (18): 4265 Figure IG3

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Fig. 3. Complementation of the S. pombe spg1-B8 and sid1-239 mutants. (A) spg1-B8 mutant cells in inducing conditions transformed with: (1) pREP3 ${Delta}$ ATG, the thiamine-repressible promoter p_nmt1 vector at 25°C (permissive temperature), and (2) at 36°C (restrictive temperature); (3) pREP3 ${Delta}$ ATG-AtSGP1 at 36°C; and (4) pREP3 ${Delta}$ ATG-AtSGP2 at 36°C. (B) sid1-239 mutant cells in inducing conditions transformed with: (1) pREP3 ${Delta}$ ATG at 25°C and (2) at 36°C; (3) pREP3 ${Delta}$ ATGBnMAP4K ${alpha}$ 2 at 36°C. Nuclei and septation material were labelled with DAPI and calcofluor, respectively. Representative examples of the septation material are indicated by arrows.