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Fig. 5. Time course of MAP2ab expression of pATSC-NS. pATSC were detached from culture dish and cultured in suspension in NB medium supplemented with B27, bFGF, EGF. Each sample was isolated at a predetermined time period, and expression of MAP2ab was analyzed by western blot and quantitated. Maximum MAP2ab levels were detected at 4 days (96 hours), after which levels were downregulated. Control pATSCs do not express the MAP2ab protein.