(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. RT-PCR analysis of stem cell and neural lineage marker expression in control pATSCs and differentiated pATSCs. Total cellular RNA (1 µg) was analyzed using the primers against several neural markers. The PCR product was separated on 1.5% agarose gel and visualized by ethidium bromide staining. Upper panel: undifferentiated pATSCs. {lambda}, molecular marker; Lane A, Oct-4; Lane B, telomerase; Lane C, Hes1; Lane D, ß-actin; Lane E, nestin; Lane F, BDNF; Lane G, MAP2ab; Lane H, GAD65; Lane I, GFAP. Lower panel: Neuronal-lineage differentiated pATSCs. Lane J, Oct-4; Lane K, nestin; Lane L, BDNF; Lane M, MAP2ab; Lane N, GAD65; Lane O, GFAP; Lane P, Trk A; Lane Q, Trk B; Lane R, P75.