Fig. 4. CAR does not interact with LIN-7b, GRIP1, or PIST. Panel A shows the immunofluorescent staining of COS-7 cells expressing CAR, LIN-7b, GRIP1-myc, or PIST-HA alone. CAR primarily localizes to the junctions between cells while LIN-7b, GRIP1, and PIST show a diffuse staining. Panel B shows no alteration in staining and no significant colocalization between LIN-7b (green), GRIP1-myc (green), or PIST-HA (green) when co-expressed with CAR (red). Panel C shows western blots of COS-7 cells co-transfected with CAR and LIN7b, GRIP1, or PIST. Each protein is detected after IP with LIN-7, myc, or HA Ab respectively but do not co-IP with CAR using the CAR-specific RmcB Ab or a nonspecific control Ab.