Fig. 4. Increased formation of G3BP-elicited SGs in the absence of AIF. Cells were treated for knock-down of AIF and then transfected with G3BP-GFP fusion constructs for 24 hours. The micrographs exemplify representative staining patterns obtained after transfection with the non-mutated G3BP-GFP construct. The histograms illustrate the quantitative difference in the formation of SG⁺ cells as a function of AIF expression and as a result of transfections with the mutant G3BP-GFP construct. Results are expressed as the percentage of SG⁺ cells exhibiting a GFP-dependent fluorescence (±s.d., n=3).