Fig. 8. Expression of recombinant lam5 rescues adhesion and survival. 3+/+, 3–/–, 3–/– expressing noncleavable or precleaved human 3 chain were plated at 10⁴ cells/well in 24-well Petri plates. Growth and deposition of the human 3 chain were monitored over a 7 day period. (A) Cell numbers were compared by counting 12 separate fields for a 10x objective. (B) ELISA for human 3 chain deposition onto the Petri dishes. (C) Phase-contrast images of cells after 7 days on Petri dishes. Bar, 25 µm. (D) Quantitation of cells in S-phase of the cell cycle after 48 hours on a Petri dish as determined by FACs analysis.