Fig. 5. The expression of Dab1RFP alters the morphology and profile of filamentous actin in NBT-II cells plated on collagen. (A) The filamentous actin distribution detected by FITC-phalloidin (green) was polarized and features of migrating cells such as lamellipodia, filopodia and stress fibers were apparent in RFP-expressing (red) NBT-II cells. This was comparable to untransfected cells (not shown). (B) Dab1RFP expression (red) resulted in a morphological change in the cell and loss of lamellipodia and stress fibers, but filopodia were apparent. (C) Expression of Dab1RFP-5F (red) did not lead to the loss of lamellipodia or stress fibers (Bar, 10 µm). Nuclei were detected by Dapi staining (blue). Arrowhead, lamellipodia; arrow, stress fibers. The cells shown are representative of the majority of cells from each transfection.