Fig. 3. Constructs harbouring kinectin and kinesin domains. cDNA fragments spanning selected sequences of human kinectin and human ubiquitous kinesin heavy chain are illustrated. They were subcloned as C-terminal fusions to the GFP ORF in vector pEGFP-C1 and used for transient transfections of HeLa cells. `Variable inserts' 1-6 in human kinectin form the `variable' C-terminal domain and their locations are illustrated (see Materials and Methods for details). The kinesin-interacting domain of kinectin is within residues 1188-1288, shared between inserts V3 and V4 (Ong et al., 2000).