Fig. 7. Effects of overexpression of kinectin-kinesin binding domains on anterograde distribution of mitochondria in HeLa cells after heat shock treatment. (A) Typical images. Mitochondria were labelled by anti-Mn SOD Ab, followed by TRITC-conjugated secondary antibody (red). Transfected cells were identified by GFP expression (green). Normal, radially distributed mitochondria, similar to those observed in non-transfected cells, appeared in GFP transfected cells (a,a'). KNT⁺ transfected cells showed pronounced clustering of mitochondria at the cell centre (b,b'). Bar, 20 µm. (B) Quantification. The values shown represent the mean±s.e.m of three experiments. A total of 90 cells were analysed per construct.