Fig. 3. ILK silencing disorganizes fibrillar adhesions. Surface distribution of the 5ß1 integrin in control and ILK siRNA-transfected cells plated on fibronectin-coated coverslips was visualized by co-staining with two different antibodies. Anti-5ß1 monoclonal (HA5, green) detects an extracellular epitope of the heterodimer and anti-5 polyclonal antibody (red) is directed against a cytoplasmic epitope of the 5 subunit. Alexa-488-congugated anti-mouse and Alexa-594-congugated anti-rabbit secondary antibodies were used; digital overlays of deconvoluted images (60x/1.40 plan Apo objective) are shown on the right. Scale bar, 20 µm.