Fig. 6. 5ß1 and vß3 integrin expression is increased in ILK-depleted cells. (A) Surface integrin expression was measured by flow cytometry. Control or ILK siRNA-transfected cells were labeled with second antibody alone (2^nd Ab), anti-5ß1 (HA5) or anti-vß3 (LM609). Quantification of specific mean of fluorescence (SMF; which corresponds to the increase in fluorescence intensity relative to second antibody alone) is shown in the table (± s.e.m., n=3 for 5ß1 integrin surface expression, n=4 for vß3 integrin expression). (B) ß1, ß3 and 5 integrin subunit expression in control or ILK siRNA-transfected cells was determined by western blotting. Total ERK1/2 levels were monitored as a control for equal protein loading. (C) ß1 and ß3 integrin mRNA expression was analysed by northern blotting. A probe for 36B4 was used to control for equal loading of RNA.