Fig. 9. Effect of ILK silencing on AKT phosphorylation and cell proliferation. (A) The levels of activated AKT and cyclin D1 expression in control and ILK siRNA-transfected cells were determined by western-blot analysis using anti-phospho-AKT (pS⁴⁷³) and anti-cyclin-D1 antibodies, respectively. Total AKT antibody was used to control for equal loading of proteins. (B) Exponentially growing control or ILK-depleted cells were fixed 36 hours after the second transfection and incubated with propidium iodide for analysis of DNA content by FACS. Results are presented as a histogram with mean values (mean ± s.e.m. from three independent experiments) corresponding to the proportion of cells in the indicated phases of the cell cycle.