Fig. 2. (A-A'') Identification of the nuclear matrix borders by immunostaining of lamins on nuclear halos immobilized on microscopic slides. (A) Nuclear halo stained with DAPI. (A') Nuclear halo immunostained using antibodies against lamins A and B. (A'') Superimposition of (A,A'). (B-B'') Hybridization in situ of Alu repeat to a nuclear halo. (B) Nuclear halo stained with DAPI. (B') Results of hybridization (immunostaining of biotinylated probe). (B'') Superimposition of (A,A').