Fig. 3. ER markers did not overlap with markers of endosomes, lysosomes, and mitochondria. (A-C) Double labeling with Texas Red-dextran (red) and GFP-tagged ER markers (green): (A) GFPKDEL, (B) GFP-SERCA2a and (C) GFP-Ins(1,4,5)P₃R1. (D-F) Double labeling with MitoTracker Red CMXRos (red) and (D) GFP-KDEL, (E) GFP-SERCA2a, and (F) GFP-Ins(1,4,5)P₃R1. The upper images are high power micrographs of the soma and the lower images are those of the dendrites. Note that most of the lysosomal and mitochondrial signals that appear to be outside of the transfected cells arise from surrounding cells. All images were taken with a confocal microscope. Scale bars: 10 µm.