Fig. 1. Localisation of histone macroH2A1.2 in surface spread spermatocytes (blue, DAPI staining; green, anti-macroH2A1.2 antibody; red, anti-SCP3 antibody; yellow/orange, co-localisation of anti-macroH2A1.2 and anti-SCP3 antibodies). (A-D) Histone macroH2A1.2 is preferentially concentrated over the forming XY-body of spermatocytes at early pachytene (lower cell) and at mid-pachytene (upper cell with DAPI dense XY-body - arrowhead in A) with a higher concentration at the X centromere (long arrow) and the synapsed PARs (short arrow) evident in the latter. (E-H) In late pachytene spermatocytes histone macroH2A1.2 is no longer concentrated throughout the XY-body, but remains concentrated on the X centromere and the PARs, and now also localises to the centromeric heterochromatin of autosomes. (Labels as for A-D.) Scale bars: 10 µm.