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Fig. 1. nimU24 strains fail to complete mitosis. Chromatin is shown in blue (DAPI staining) and spindle pole bodies in pink (indirect immunofluorescence with anti-{gamma} tubulin antibody). (A) A nimU24 cell (EM4) with multiple separated spindle pole bodies (SPBs) but foci of chromatin connected by strands of chromatin. In this image the chromatin appears condensed. (B) A uninucleate nimU24 cell (EM4) with two separated SPBs. (C) A wild-type cell (GR5) containing two normal interphase nuclei, each with a single SPB. (D) SPB separation was scored in a nimU24 strain (EM4) and a wild-type strain (GR5) through the time course indicated, at the restrictive temperature (42°C). Scale bar: 5 µm.