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Fig. 2. Mitotic failure in the absence of nimU function. (A) Chromosome mitotic index was scored in wild-type (GR5), nimU24 (EM4), and nimU{Delta} (GR5/{Delta}15) cells at nimU24 permissive and restrictive temperatures. (B) After 10 hours at the restrictive temperature a typical wild-type germling has undergone three mitoses to produce eight nuclei (top panel). Under the same conditions a nimU24 germling has an enlarged, misshapen nucleus (lower panel).