Fig. 6. The spindle assembly checkpoint cannot delay mitotic exit in the absence of nimU. (A) The G2/M block/release experiment was repeated in the presence of benomyl and the chromosome mitotic index determined. (B) Conidiospores were spot-inoculated onto rich medium with or without benomyl and germinated at semi-permissive (32°C) or restrictive (34.5°C) temperatures for the nimU24 mutant for several days. sldA744 and sldB937 are loss of function mutations in the spindle checkpoint components BUB1 and BUB3, respectively.