Fig. 9. Sperm function correlates with tyrosine phosphorylation. (A) Cells were placed into either complete BWW (lane 1) or BWW -Ca²⁺ (lane 2). Approximately 2.5 hours later, an aliquot of cells in BWW -Ca²⁺ was placed into complete BWW (lane 3) and challenged with 2.5 µM A23187 for a further 30 minutes. The level of acrosome reaction was then counted as described in Materials and Methods. The graph represents the mean and standard deviation of three independent experiments; ^*P<0.05. (B) Phosphotyrosine expression in the treatments illustrated in A. (C) The nitrocellulose membrane was stripped at 65°C with shaking. Following this, the membrane was blocked and re-probed using -tubulin antibody. The western blot is representative of three independent experiments.