Fig. 4. Tropomyosin is absent from early podosomes. (A-C) Similar to the situation with myosin II, TM is rearranged and diffused at the sites of cortactin clustering during the early phase of podosome formation. Higher magnification of a selected area is shown in A'-C'. Arrowheads indicate regions of TM dispersion and podosome formation. (D-F) Only at later stages are tropomyosins found at, and overlap with, the periphery of larger podosomes. Scale bar: 20 µm.