Fig. 2. Major structural elements of the usherin protein based on amino acid sequence. The amino acid positions where domains start and end are indicated. The location of peptides used to derive antibodies 1 and 2 used in these studies are shown. Constructs used to generate fusion peptides comprised the indicated portions of the thrombospondin 1, LN, LE and fibronectin type III domains (Ts-FP, LN-FP, LE-FP, and FNFP, respectively).