Fig. 6. The expression of p120 in epithelial SW48 cells is sufficient to induce cell spreading on hEcad-Fc and cadherin adhesions. (A) E-cadherin-expressing SW48 cells grown in standard conditions and immunostained for p120 and ß-catenin. (B) Equal quantities (10 µg) of proteins extracted from SW48 cells transfected with GFP (lane 1) or p120-GFP (lane 2) were separated on 7% SDS-PAGE and immunoblotted with anti-p120, anti-GFP, anti-ß-catenin antibodies, then with anti-tubulin antibodies as a loading control. (C) GFP- and p120-GFP-expressing SW48 cells were plated on hEcad-Fc (/hEcad-Fc) for 2 hours and immunostained for ß-catenin. While GFP-expressing cells behave exactly as GFP-negative cells (top row), p120-GFP SW48 cells spread extensively on the hEcad-Fc substrate (second row). Concomitantly, all these spread cells organized ß-catenin-positive radial structures in their lamellipodium (enlargement, third row). By contrast, p120-transfected SW48 cells did not spread better than untransfected cells on fibronectin (/Fibronectin). Bar, 10 µm.