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Fig. 7. Cell spreading and cadherin adhesions are inhibited by p120 overexpression in C2 cells. (A) C2 cells expressing p120-GFP alone or together with DA Rac1 were plated on Ncad-Fc for 2 hours before labelling with anti-ß-catenin antibodies. (B) Cells were double transfected with p120-GFP and the Myc-tagged JMD-cad construct, then seeded on Ncad-Fc for 2 hours and labelled with anti-ß-catenin and anti-Myc antibodies. Bar, 10 µm. (C) The histogram presents the quantification of cadherin adhesions in the various conditions, expressed as a percent of the control conditions. At least 200 cells were counted in two independent experiments.