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Fig. 5. Cell cycle distribution showing differential patterns induced by TAp73{alpha} overexpression. (A) FACS. (B) Histogram representation of cell cycle population: Sub-G1 (black columns); G1 (white columns); S (hatched); G2/M (dots); data are means of three independent experiments ± s.d. (bars). The proportion of Ad-TAp73 SH-SY5Y neuroblasts (Ad-p73{alpha}) that underwent Sub-G1 phase increased considerably (60%) compared to Ad-{Delta}Np73{alpha} infected cells (10%) and to the non-infected cells and cells infected by empty vector (5% and 9%, respectively), indicating cells entering apoptosis. Ad-TAp73 IGR-N-91 cells exhibited a cell cycle distribution different from that of SH-SY5Y cells: more of the population underwent G1 phase (62% in treated versus 48% in control) leading to cell cycle arrest mediated by p21 activation as confirmed by western blot analysis shown in Fig. 3A.