Fig. 5. Percentages of PVs able to accumulate the weak base DAMP or containing various host cell molecules from the endocytic route in Leishmania-infected DCs. Twenty-one to 26 hours after infection with amastigotes from nude mice (Am nude; white bars) or metacyclic promastigotes (MPm Ficoll; grey bars), DCs were fixed, permeabilised and incubated with immunological reagents before analysis by fluorescence microscopy. Association of the following molecules with parasite-containing parasitophorous vacuoles (PVs) was examined: lamp-1, lamp-2, rab7p, MHC Class II and H-2M molecules. To determine the association of 3-(2,4-dinitroanilino)-3' amino-N-methyldipropylamine (DAMP) with PVs, infected DCs were processed as described in the legend of Fig. 4. About 100-200 organelles were counted for each molecule examined. Results are representative of two or three experiments.