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Fig. 1. {alpha}1,6-Mannosylation of pro-CPY in the ER. Sec13-1 (lanes 1 and 2; H230), sec18-1 (lanes 3; H4), sec23-1 (lanes 4; H238) and {Delta}och1 (panel C, H1691) were preincubated for 15 minutes, pulse-labelled with [35S]methionine/cysteine for 5 minutes and chased in the presence of CHX for 30 minutes (A,B) or 20 minutes (C) at the indicated temperatures. The cell lysates were subjected to immunoprecipitation with CPY antiserum alone, or first with CPY antiserum followed by reimmunoprecipitation with antiserum against {alpha}1,6-mannose residue as indicated, followed by SDS-PAGE analysis. The untranslocated form (pre), ER form (p1), Golgi form (p2) and mature CPY (m) are indicated on the right.