JCS -- Karhinen and Makarow 117 (2): 351 Figure IG3

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Fig. 3. Elongation of O-glycans of Hsp150 ${Delta}$ -ß-lactamase in the ER. Sec13-1 (lanes 1-12; H1065), or sec18-1 (lanes 13-16; H393) mutants were preincubated for 15 minutes, pulse labelled for 5 minutes with [³⁵S]methionine/cysteine (lanes 1-8 and 13-16) or [³H]mannose (lanes 9-12), and chased with CHX as indicated. The cell lysates (c; lanes 1-4 and 9-16) and culture media (m; lanes 5-8) were subjected to immunoprecipitation with ß-lactamase antiserum followed by SDS-PAGE analysis. The migration of the various Hsp150 ${Delta}$ -ß-lactamase forms is indicated on the right, and that of molecular weight markers on the left.