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Fig. 7. Immunofluorescent staining of Och1p-HA. Sec23-1 cells (H1490; A,B), sec7-1 cells (H1489; C), or normal cells (H1488; D) were grown at 24°C and fixed immediately (A,D), or incubated with CHX for 1 hour at 24°C (B), or thereafter for an additional 30 minutes at 37°C (C) before fixation. The cells were subjected to indirect immunofluorescent staining with antibody against the HA epitope (left), and DAPI (right).