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Fig. 3. HA mediates the expression of Mmp-9 through interaction with a membrane receptor. MEFs were either treated or not with Suramin before stimulation with rooster comb HA. As a control, MEFs were treated with TNF{alpha}. Total RNA was prepared and analysed for Mmp-9 and Mmp-13 transcripts by RT-PCR. RT-PCR of GAPDH mRNA was carried out to ensure equal cDNA amounts in the different samples.