Fig. 8. The number of new nerve sprouts was increased by the wound-induced EF. From 16 to 24 hours after wounding, new sprouts grew from the intact nerves (compare 16 and 24 hour control values, P<0.01). Pharmacologically enhancing the endogenous EFs also significantly increased new sprouts as early as 16 hours (compare 16 hour control values with values for all other treatments at 16 hours, P<0.01). Ouabain, furosemide, neomycin and d-tubocurare all reduced nerve sprout numbers 24 hours after wounding (when compared with 24 hour control value).