Fig. 2. Expression of Gie in various human tissues and cell lines. (A) Expression of hGie1 and hGie2 mRNAs in human tissues. Northern blots of poly(A)⁺ RNAs from various human tissues (Clontech) were hybridized with ³²P-labeled Gie probes. (B) Extracts from PC12 cells were separated by SDS-PAGE and immunoblotted with pre-immune serum (left), the anti-Gie antibody (middle) or the same antibody that had been incubated with the antigen peptide (right). (C) Extracts from the indicated cell lines were also immunoblotted with the anti-Gie (top) and anti-GAPDH (bottom) antibodies.