Fig. 5. Cell-cycle-dependent dynamics of Gie localization. PC12 cells were fixed and stained for endogenous Gie with the affinity-purified anti-Gie antibody (green). Microtubules (red) and DNA (blue) were also visualized with anti--tubulin antibody and DAPI, respectively. Gie displayed cytoplasmic distribution in interphase (white arrows) but moved prominently to the spindle mid-zone (yellow arrows) in anaphase and to the mid-body in late-telophase (yellow arrowhead). Scale bar, 5 µm.