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Fig. 7. Overexpression of Gie1 mutants lacking the switch regions impairs chromosome segregation in HeLa cells. (A) HeLa cells that had been transfected with the indicated Gie1 and cultured for 24-30 hours were analysed by immunofluorescence microscopy. DNA (green) and Gie1 (red) were detected with PicoGreen and the anti-Gie antibody, respectively. Impaired chromosome segregation was observed in cells expressing Gie1/{Delta}31-60 and Gie1/{Delta}61-90 (white arrows). Scale bar, 5 µm. (B) The appearance of abnormal nuclei was measured and the data are represented as percentages of means±s.e.m. from at least three independent experiments (each of 200-400 cells).