Fig. 1. Structure and expression of HERC5. (A) Schematic domain structure of HERC5. Amino acids in Croco different from those in Ceb1 are shown as grey bars. aa, amino acid; HECT, homologous to E6-AP C-terminus; RLD, RCC1-like domain. (B) Comparison of Croco and Ceb1. Differences in the cDNA and protein sequences are indicated in bold. Amino acids are given in the single-letter code and positions of amino acids are indicated below. GenBank accession numbers: Ceb1, AB027289; Croco, AY337518. (C) HERC5 mRNA expression in human tissues. A commercially available multiple tissue blot containing poly(A)+ RNA derived from different human tissues was probed for HERC5 transcripts. sk. muscle, skeletal muscle. (D) Quantification of HERC5 mRNA in selected tissues. Quantitative real-time RT-PCR was performed to quantify HERC5 expression in heart, placenta and testis normalized to ß2-microglobulin expression.