Fig. 1. Gyp5p and Gyl1p localize at the bud tip during polarized growth, at the bud neck during cytokinesis, and are partially colocalized. (A) Yeast cells expressing Gyp5p-Myc (green) and Gyl1p-HA (red) were stained by indirect immunofluorescence and observed with a 3D deconvolution microscopy system. Images shown are projections of deconvoluted Z-series combining green and red signals. Bars, 1 µm. White squares indicate the regions magnified in B. (B) Single sections of part of each image in A. Bars, 1 µm.