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Fig. 7. gyp5{Delta}gyl1{Delta} cells display a cold-sensitive slow growth phenotype and a frequent accumulation of secretory vesicles in small buds. (A) Doubling time of WT and gyp5{Delta}gyl1{Delta} strains in rich medium was determined in early log phase at 13° and 30°C. Values are the average of results obtained with four independent clones. (B) Thin section electron microscopy was performed on WT and gyp5{Delta}gyl1{Delta} cells cultured at 13°C for 16 hours. Small buds correspond to a ratio (bud width/bud neck width) <2, large buds to a ratio of 2 and higher. The % of WT cells and gyp5{Delta}gyl1{Delta} cells containing at least five distinct secretory vesicles were determined. (C) Thin section electron microscopy of WT (a) and gyp5{Delta}gyl1{Delta} cells (b-d). Bars, 500 nm.