JCS -- Win et al. 117 (20): 4769 Figure IG5

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Fig. 5. top3-134 is synthetically lethal in combination with deletion of rad3/rad26 but not with deletion of other checkpoint genes. (A-D) Tetrads derived from diploid strains of the following genotypes were microdissected onto YES agar: (A) h⁺/h^- rad3::ura4⁺/rad3⁺ top3-134/top3⁺; (B) h⁺/h^- rad26::ura4⁺/rad26⁺ top3-134/top3⁺; (C) h⁺/h^- rad1::ura4⁺/rad1⁺ top3-134/top3⁺; and (D) h⁺/h^- rad17::ura4⁺/rad17⁺ top3-134/top3⁺. Colonies resulting from nine tetrads in each case were photographed after seven days growth at 26°C. The genotypes of the segregants were determined by replica plating and are indicated schematically [right: (A) W, top3⁺ rad1⁺ rad3⁺ rad17⁺ rad26⁺; T, top3-134, R, ${Delta}$ rad3; (B) ${Delta}$ rad26; (C) ${Delta}$ rad1; or (D) ${Delta}$ rad17; (A) RT, top3-134 ${Delta}$ rad3; (B) top3-134 ${Delta}$ rad26; (C) top3-134 ${Delta}$ rad1; (D) or top3-134 ${Delta}$ rad17]. Boxes (left) indicate the position of top3-134 double mutants with rad1, rad3, rad17 and rad26, respectively.