Fig. 7. Aberrant nucleolar structures in the top3-134 mutant. (A) Living gar2-GFP (top3⁺) and gar2-GFP top3-134 (top3-134) cells were observed by green fluorescence microscopy. Arrowheads indicate cells with aberrant nucleolar structures. (B) Merged images (lower panels) of fluorescence micrographs showing Gar2-GFP (upper panels) and DNA (Hoechst 33342, middle panels) localisation in living cells. (C) Visualisation of lagging Gar2-GFP signal in top3-134 cells. Individual gar2-GFP and gar2-GFP top3-134 cells were observed as in A, over a 20 minute period, with images collected every 4 minutes. Bar, 10 µm.