Fig. 5. (A) The translocation of G_q is independent of TRPL and Arrestin-2. Cross-sections from trpl³⁰² and arr2⁵ null mutants were stained with an antibody for G_q. All flies were either dark-raised or light exposed for 2 hours. G_q displayed normal, light-dependent translocation in both the trpl and the arr2 mutants, indicating their non-involvement in G_q's translocation. (B) The translocation of TRPL and Arr2 is independent of G_q. Cross-sections from dark-raised and light-exposed dgq¹ mutants were immunostained for TRPL and Arr2. The dgq¹ mutant is a severe hypomorph that produces 1% of the wild-type levels of G_q (Scott et al., 1995). TRPL displays normal translocation, moving from the rhabdomere to the cell body upon illumination. Likewise, Arr2 displays normal light-dependent translocation from the cell body to the rhabdomere. These results indicate that the light-regulated translocation of both Arrestin2 and TRPL do not require G_q.