Fig. 6. The mechanism of G_q translocation does not involve Shibere-mediated endocytosis. Dark-raised shibere^ts1 flies were first incubated at 30°C for 1-2 minutes in the dark to block endocytosis, as indicated by paralysis. Paralyzed shibere^ts1 flies continued to be incubated at 30°C but were either kept in the dark or transferred to the light for 1 hour. This incubation did not harm the flies because paralysis was reversible. Cross-sections were stained for G_q. When endocytosis is disrupted, G_q still displays light-regulated translocation from the rhabdomere to the cell body.