Fig. 2. Gene expression in wild type and var3. (A) RNA blot of 20 µg total RNA from wild type (wt) and var3 probed with labeled cDNAs of CAB (X56062), RbcS (X13611; Lhcb1) and EF-1 (16260) control. (B) RT-PCR detection of Lhcb1 in wt and var3 under nonphoto-oxidative conditions (green, wt_g and var3_g) and photo-oxidative conditions (5 µM norflurazon, wt_Nf and var3_Nf) compared with EF-1 control. (C) Scatter plot of z-score of slide 1 versus z-score of slide 2 (z-score=(int_var3-int_wt)/std_14wt). Dots in first and third quadrants represent consistent measurements of clones from both arrays from which the var3 sample was measured higher or lower relative to wt, respectively. Dots in second and fourth quadrants represent inconsistent measurements. The line represents a geometric mean of 4 std., equal to the most extreme, inconsistent measurement (geometric mean of absolute values). (D) The table shows the top genes ranked after the geometric mean of z-scores. C, chloroplast encoded; M, mitochondrion encoded; N, nuclear encoded; NC, nuclear encoded chloroplast targeted. ^*The protein was formerly designated HSP70-7 but is now known as Hsc70-2 (Sung et al., 2001).