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Fig. 3. VAR3 locus and mRNA. (A) VAR3 wild type, sequence of exon one (Var3), sequence of var3 allele with Ds left border (in gray) (var3 left) and var3 with Ds right border (gray) and the 8-bp repeat underlined (var3 right). Numbers give nucleotide position from the same sequence of wild type Col-O (complement of P1 clone MVA3, AB006706). (B) RT-PCR detection of VAR3 mRNA in wild type (wt) and var3 (lanes 2 and 4) compared to control EF-1{alpha} mRNA (lanes 1 and 3). Molecular markers are shown on both sides of the gel.