Fig. 9. 3XALA cells exhibit aberrant streaming and also have problems entering streams of control cells. (A) Streaming during late aggregation of JH10 cells on agar. (B) Streaming during late aggregation of mhcA-/mhcA+ cells on agar. (C) Late aggregation of 3XALA cells on agar. (D) DiI-labeled 3XALA cells (red) mixed with unlabeled JH10 cells at a ratio of 1:9 failed to enter JH10 streams. Stained and unstained cells were co-imaged with simultaneous bright-field optics and laser scanning confocal microscopy.