Fig. 3. Autophagic vacuoles were frequently detected in the perinuclear area close to the Golgi apparatus. HeLa cells were starved for 2 hours to induce autophagy and prepared for immunogold labelling of LC3 (A) or Lamp1 (B) followed by a secondary antibody coupled to 5 nm gold (arrowheads). Autophagic vacuoles (AV) were frequently observed to accumulate close to the nucleus (Nu) and the Golgi stack (G), where endo/lysosomal vacuoles (LE) were also present. Some autophagic vacuoles were observed to fuse with the Lamp1-positive endo/lysosomal vacuoles (B).