Fig. 2. v-Src increases spontaneous motility in Dunn chambers. To analyse individual movement, cells were plated at low density on glass slides. These were placed in Dunn chambers in the absence of added growth factor and phase-contrast images were recorded at 2-minute intervals. (A) Trajectories during 7 hours of migration; circles indicate initial positions. Scale bar: 200 µm (see also Movies 1-4, supplementary material). (B) Mean speed of migration: data were pooled from 3-4 experiments (n=150 to 180 cell trajectories) in total; box plots represent median (bold vertical line), interquartile range (boxes) and full values range (extremes); ^***P<0.001 by Mann-Whitney test.