Fig. 1. Tiam1 expression induces an ALCAM-mediated epithelial-like morphology in metastatic melanoma cells. (A) Western blot analysis of parental and Tiam1-expressing melanoma cell lines. Lysates from parental and Tiam1-expressing 1F6 and MV3 cells were immunoblotted with Tiam1 and ALCAM antibodies. ß-Actin was used as loading control to ensure equal loading. (B) Phase-contrast images of melanoma cell lines. (C) Confocal images of melanoma cell lines stained for Tiam1 (green), ALCAM (white) and F-actin (red). Note that, in MV3-Tiam1 cells, ALCAM is strongly clustered at cell-cell contacts, which is accompanied by distinct colocalization of Tiam1, F-actin and ALCAM. Bars, 20 µm.