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Fig. 5. RPA reassembles to pre-replication foci in high-speed egg extracts. (A) Experimental scheme. Sperm nuclei were incubated as described in legend to Fig. 4 except that cycle 2 was followed both in a low-speed egg extract (LSE) and a high-speed egg extract (HSE). (B) The assembly of RPA34 was analyzed at the entry in a new interphase in LSE or HSE, as in legend to Fig. 4.