Fig. 7. Short nascent DNAs are synthesized in MCM3-depleted extracts. Alkaline agarose gel electrophoresis of DNA synthesized in egg extracts depleted with control mouse IgGs (mock-depleted), anti-MCM3 (MCM3-depleted) (A) or Cdt1 (Cdt1-depleted) antibodies (B). Depletion of the Cdt1 protein was over 98% (data not shown).