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Fig. 1. Immunoblot analysis of Ncd and KLP61F expression. (A) Crude extracts of ovaries of wild-type females probed with antibodies from immune (I) and preimmune (P) antibodies against Ncd. (B) Crude extracts of larval brains of wild type (wt), ncd1 and klp61F4 ncd1 mutants fractionated in three wide lanes (underscores) of a 10% SDS-PAGE gel. Parallel channels of an Immunetics manifold were probed with antibodies against Ncd (Ab Ncd) and KLP61F (Ab KLP61F). Positions of Ncd (*) and KLP61F (**) proteins are indicated.