Fig. 3. Nuclear lamina organization in klp61F and klp61F ncd mutants. Larval brains of wild type (A) and klp61F³ (B), klp61F³ ncd¹/Df(3L)bab PG (C) and klp61F³ ncd¹/klp61F⁴ (D) mutants stained with antibodies against lamins (grayscale), -tubulin (red) and a chromatin dye (blue). Arrows in (B, C) indicate involutions of the nuclear lamina that extend toward centrosomes. (D) Cell with bipolar positioning of centrosomes and metaphase alignment of chromosomes, showing regions of (arrow) disorganization and blebbing of nuclear lamina. Images of -tubulin and chromatin are single projected images of a stack of deconvolved sections. Images of lamin staining are single deconvolved sections chosen to show involutions in nuclear lamina. Bar, 5 µm.